Consuelo Sauceda
University of California, San Diego
San Diego, CA
The proteomic atlas of the gut commensal Phocaeicola vulgatus uncovers key neutrophil antimicrobial peptide resistance proteins with previous unknown function
Consuelo Sauceda, Carlos G. Gonzalez, Igor H. Wierzbicki, Jessica Raygoza
Gut commensal, Phocaeicola vulgatus, is among the most abundantly isolated human microbes with increasing research showing its role in inflammatory bowel disease (IBD). To better comprehend host-microbe interactions related to IBD it is important to understand the molecular repertoire used by P. vulgatus in the gut environment. To date, the functional components (i.e. the proteome) of P. vulgatus elaborates during stress conditions associated with IBD remain a mystery. To fill this knowledge gap, we combined advanced mass-spectrometry-based proteomics and clinically relevant neutrophil antimicrobial peptide challenges, to provide a multidimensional proteome atlas of the P. vulgatus cell. Our proteome atlas includes the architecture of the whole P. vulgatus cell, its surface-shavings (surfome), and secreted (secretome) components. Our proteomics atlas corroborated the identification of differentially expressed protein networks of functional categories involved in antimicrobial stress response and guided the discovery of proteins with previously unknown functions associated with gut cell damage.
SACNAS National Diversity in STEM Conference, Phoenix, AZ, October 30-November 2, 2024
