2021 SACNAS Graduate Student Oral Presentation Award (Dominguez)

Sedelia Dominguez
Washington State University
Pullman, WA

F. tularensis exploits AMPK activation to acquire host-derived nutrients

Francisella tularensis, a highly virulent bacterial pathogen, causes tularemia in humans. In humans, tularemia leads to acute infections, but chronic infections result in bacteremia and have a high chance of being fatal. With a low infectious dose and potential to be used as a bioterrorism weapon, F. tularensis poses a significant public health risk. During infection, F. tularensis encounters a wide range of environments within cells. Following entry into the cell and phagosome escape, the bacterium replicates rapidly in the cell cytoplasm. F. tularensis replication depends on the availability and use of various cellular nutrients. However, the mechanisms by which these nutrients become available to the bacterium is not fully understood. We found that F. tularensis infected cells had increased activation of adenosine monophosphate protein kinase (AMPK). Activated AMPK increased lipid degradation in infected cells. An increase in lipid droplet degradation allows F. tularensis to use carbon sources, such as fatty acids and glycerol, as energy for replication. Our findings suggest F. tularensis exploits AMPK activation to access nutrients sequestered in lipid droplets to undergo efficient bacterial replication that leads to a successful infection.

SACNAS Virtual National Diversity in STEM Conference, October 25-29, 2021

  1. Dominguez, SR, Doan, PN, Rivera-Chávez, F. The intersection between host-pathogen interactions and metabolism during Vibrio cholerae infection. Curr Opin Microbiol 2024; 77 : 102421. PubMed PMID:38215547 .
  2. Dominguez, SR, Whiles, S, Deobald, KN, Kawula, T. Francisella tularensis Exploits AMPK Activation to Harvest Host-Derived Nutrients Liberated from Host Lipolysis. Infect Immun 2022; 90 (8): e0015522. PubMed PMID:35916521 PubMed Central PMC9387300.
  3. Dominguez, SR, Kawula, T, Steele, SP. Bacterial Synchronized Transfer Assays in Bone Marrow Derived Macrophages. Bio Protoc 2019; 9 (22): . PubMed PMID:33117859 PubMed Central PMC7591151.
Search PubMed

Posted in Uncategorized and tagged .

Dana Crawford

Professor of Population and Quantitative Health Sciences and Associate Director of the Cleveland Institute for Computational Biology, with interest in pharmacogenomics, electronic health records, and diverse populations. Also, an avid foodie!